ptriex rhoa fret wt biosensor Search Results


addgene plasmid  (Addgene inc)
92
Addgene inc addgene plasmid
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptriex rhoa wt mscarlet i sgfp2  (Addgene inc)
91
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Ptriex Rhoa Wt Mscarlet I Sgfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptriex rhoa wt mscarlet i sgfp2 - by Bioz Stars, 2026-07
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ptriex rhoa flare sc biosensor t19n  (Addgene inc)
93
Addgene inc ptriex rhoa flare sc biosensor t19n
Fig. 5 Inflammation disrupts Ca2+, pH, and mitochondrial homeostasis in RhoA-deficient microglia. A Lifeact fluorescence labeling in RhoA WT, RhoA <t>T19N,</t> or RhoA KO HMC3 microglia (n = 3 independent experiments). B Cell counting in RhoA WT, RhoA Q63L, RhoA T19N, or RhoA KO HMC3 microglia (n = 8 independent experiments). Graph displays mean with SD. ****p < 0.0001 (Two-way ANOVA). RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia expressing a global Ca2+ biosensor (C), mitochondrial Ca2+ biosensor (D), endoplasmic reticulum Ca2+ biosensor (E), pH biosensor (F), MitoTimer biosensor (G), or mitophagy biosensor (H) and exposed to LPS (1 µg/ml; 20 min (C-F) or 60 min (G and H)). Graphs (mean and SD) display fluorescence changes (n = 15 cells per group from 3 independent experiments for each biosensor). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.
Ptriex Rhoa Flare Sc Biosensor T19n, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptriex+rhoa+fret+wt+biosensor/pm37863874-387-16-20?v=Addgene+inc
Average 93 stars, based on 1 article reviews
ptriex rhoa flare sc biosensor t19n - by Bioz Stars, 2026-07
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ptriex rhoa flare sc biosensor wt  (Addgene inc)
93
Addgene inc ptriex rhoa flare sc biosensor wt
Fig. 5 Inflammation disrupts Ca2+, pH, and mitochondrial homeostasis in RhoA-deficient microglia. A Lifeact fluorescence labeling in RhoA WT, RhoA <t>T19N,</t> or RhoA KO HMC3 microglia (n = 3 independent experiments). B Cell counting in RhoA WT, RhoA Q63L, RhoA T19N, or RhoA KO HMC3 microglia (n = 8 independent experiments). Graph displays mean with SD. ****p < 0.0001 (Two-way ANOVA). RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia expressing a global Ca2+ biosensor (C), mitochondrial Ca2+ biosensor (D), endoplasmic reticulum Ca2+ biosensor (E), pH biosensor (F), MitoTimer biosensor (G), or mitophagy biosensor (H) and exposed to LPS (1 µg/ml; 20 min (C-F) or 60 min (G and H)). Graphs (mean and SD) display fluorescence changes (n = 15 cells per group from 3 independent experiments for each biosensor). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.
Ptriex Rhoa Flare Sc Biosensor Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptriex+rhoa+fret+wt+biosensor/pmc07949103-332-11-22?v=Addgene+inc
Average 93 stars, based on 1 article reviews
ptriex rhoa flare sc biosensor wt - by Bioz Stars, 2026-07
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resource source identifier plncx chick src y527f joan brugge unpublished rrid addgene 13660 plncx chick src joan brugge  (Addgene inc)
93
Addgene inc resource source identifier plncx chick src y527f joan brugge unpublished rrid addgene 13660 plncx chick src joan brugge
Fig. 5 Inflammation disrupts Ca2+, pH, and mitochondrial homeostasis in RhoA-deficient microglia. A Lifeact fluorescence labeling in RhoA WT, RhoA <t>T19N,</t> or RhoA KO HMC3 microglia (n = 3 independent experiments). B Cell counting in RhoA WT, RhoA Q63L, RhoA T19N, or RhoA KO HMC3 microglia (n = 8 independent experiments). Graph displays mean with SD. ****p < 0.0001 (Two-way ANOVA). RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia expressing a global Ca2+ biosensor (C), mitochondrial Ca2+ biosensor (D), endoplasmic reticulum Ca2+ biosensor (E), pH biosensor (F), MitoTimer biosensor (G), or mitophagy biosensor (H) and exposed to LPS (1 µg/ml; 20 min (C-F) or 60 min (G and H)). Graphs (mean and SD) display fluorescence changes (n = 15 cells per group from 3 independent experiments for each biosensor). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.
Resource Source Identifier Plncx Chick Src Y527f Joan Brugge Unpublished Rrid Addgene 13660 Plncx Chick Src Joan Brugge, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptriex+rhoa+fret+wt+biosensor/pm32579923-263-2-12?v=Addgene+inc
Average 93 stars, based on 1 article reviews
resource source identifier plncx chick src y527f joan brugge unpublished rrid addgene 13660 plncx chick src joan brugge - by Bioz Stars, 2026-07
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ptriex rhoa flare sc biosensor q63l  (Addgene inc)
91
Addgene inc ptriex rhoa flare sc biosensor q63l
Fig. 2 RhoA regulates microglial metabolic reprogramming during inflammation. HMC3 microglia expressing the ATP biosensor (A), Glucose biosensor (B), Lactate biosensor (C), or Pyruvate biosensor (D) were transfected with RhoA <t>Q63L</t> (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 15-30 cells per group from 3 independent experiments for each biosensor). Primary cortical microglia expressing the ATP biosensor (E) or Lactate biosensor (F) were transfected with the RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 6 cells per group from 3 independent experiments for each biosensor). Panels are time-lapse ratio images coded according to the pseudocolor ramps. Graphs (means and SD) display F490/F435 (A and E), FRET/Donor (B), and Donor/FRET (C, D, and F) ratio change at 0 (CT) and 20 min. G Seahorse measurements of bioenergetic parameters in HCM3 microglia expressing RhoA Q63L or RhoA WT. The parameters were calculated based on the OCR following the sequential addition of LPS, oligomycin, FCCP, rotenone, and antimycin A. Results are from at least 3 independent experiments. Graphs show the mean with SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.
Ptriex Rhoa Flare Sc Biosensor Q63l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptriex+rhoa+fret+wt+biosensor/pm37863874-387-11-15?v=Addgene+inc
Average 91 stars, based on 1 article reviews
ptriex rhoa flare sc biosensor q63l - by Bioz Stars, 2026-07
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ptriex rhoa 2g  (Addgene inc)
93
Addgene inc ptriex rhoa 2g
Fig. 2 RhoA regulates microglial metabolic reprogramming during inflammation. HMC3 microglia expressing the ATP biosensor (A), Glucose biosensor (B), Lactate biosensor (C), or Pyruvate biosensor (D) were transfected with RhoA <t>Q63L</t> (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 15-30 cells per group from 3 independent experiments for each biosensor). Primary cortical microglia expressing the ATP biosensor (E) or Lactate biosensor (F) were transfected with the RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 6 cells per group from 3 independent experiments for each biosensor). Panels are time-lapse ratio images coded according to the pseudocolor ramps. Graphs (means and SD) display F490/F435 (A and E), FRET/Donor (B), and Donor/FRET (C, D, and F) ratio change at 0 (CT) and 20 min. G Seahorse measurements of bioenergetic parameters in HCM3 microglia expressing RhoA Q63L or RhoA WT. The parameters were calculated based on the OCR following the sequential addition of LPS, oligomycin, FCCP, rotenone, and antimycin A. Results are from at least 3 independent experiments. Graphs show the mean with SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.
Ptriex Rhoa 2g, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptriex+rhoa+fret+wt+biosensor/bio_rxiv__2024__07__28__605413-213-25-32?v=Addgene+inc
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ptriex rhoa 2g - by Bioz Stars, 2026-07
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ptriex mcherry zdk1 rhoa q63l  (Addgene inc)
93
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Fig. 2 RhoA regulates microglial metabolic reprogramming during inflammation. HMC3 microglia expressing the ATP biosensor (A), Glucose biosensor (B), Lactate biosensor (C), or Pyruvate biosensor (D) were transfected with RhoA <t>Q63L</t> (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 15-30 cells per group from 3 independent experiments for each biosensor). Primary cortical microglia expressing the ATP biosensor (E) or Lactate biosensor (F) were transfected with the RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 6 cells per group from 3 independent experiments for each biosensor). Panels are time-lapse ratio images coded according to the pseudocolor ramps. Graphs (means and SD) display F490/F435 (A and E), FRET/Donor (B), and Donor/FRET (C, D, and F) ratio change at 0 (CT) and 20 min. G Seahorse measurements of bioenergetic parameters in HCM3 microglia expressing RhoA Q63L or RhoA WT. The parameters were calculated based on the OCR following the sequential addition of LPS, oligomycin, FCCP, rotenone, and antimycin A. Results are from at least 3 independent experiments. Graphs show the mean with SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.
Ptriex Mcherry Zdk1 Rhoa Q63l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptriex+rhoa+fret+wt+biosensor/bio_rxiv__2025__09__23__677979-161-13-15?v=Addgene+inc
Average 93 stars, based on 1 article reviews
ptriex mcherry zdk1 rhoa q63l - by Bioz Stars, 2026-07
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ptriexrhoa2g (plasmid #40176)  (Addgene inc)
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ptriex-mvenus-zdk1-rhoa q63l (plasmid #81046)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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ptriex-rhoa-q63l_mscarlet-i_sgfp2 (plasmid #85072)  (Addgene inc)
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ptriex-mvenus-pi-rhoa(q63l) (plasmid #86978)  (Addgene inc)
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Image Search Results


Fig. 5 Inflammation disrupts Ca2+, pH, and mitochondrial homeostasis in RhoA-deficient microglia. A Lifeact fluorescence labeling in RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia (n = 3 independent experiments). B Cell counting in RhoA WT, RhoA Q63L, RhoA T19N, or RhoA KO HMC3 microglia (n = 8 independent experiments). Graph displays mean with SD. ****p < 0.0001 (Two-way ANOVA). RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia expressing a global Ca2+ biosensor (C), mitochondrial Ca2+ biosensor (D), endoplasmic reticulum Ca2+ biosensor (E), pH biosensor (F), MitoTimer biosensor (G), or mitophagy biosensor (H) and exposed to LPS (1 µg/ml; 20 min (C-F) or 60 min (G and H)). Graphs (mean and SD) display fluorescence changes (n = 15 cells per group from 3 independent experiments for each biosensor). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.

Journal: Cell death & disease

Article Title: RhoA balances microglial reactivity and survival during neuroinflammation.

doi: 10.1038/s41419-023-06217-w

Figure Lengend Snippet: Fig. 5 Inflammation disrupts Ca2+, pH, and mitochondrial homeostasis in RhoA-deficient microglia. A Lifeact fluorescence labeling in RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia (n = 3 independent experiments). B Cell counting in RhoA WT, RhoA Q63L, RhoA T19N, or RhoA KO HMC3 microglia (n = 8 independent experiments). Graph displays mean with SD. ****p < 0.0001 (Two-way ANOVA). RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia expressing a global Ca2+ biosensor (C), mitochondrial Ca2+ biosensor (D), endoplasmic reticulum Ca2+ biosensor (E), pH biosensor (F), MitoTimer biosensor (G), or mitophagy biosensor (H) and exposed to LPS (1 µg/ml; 20 min (C-F) or 60 min (G and H)). Graphs (mean and SD) display fluorescence changes (n = 15 cells per group from 3 independent experiments for each biosensor). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.

Article Snippet: Raichu-RhoA (provided by M. Matsuda [27]), pTriEx-RhoA FLARE.sc Biosensor WT (RRID:Addgene_12150), pTriEx-RhoA FLARE.sc Biosensor Q63L (RRID:Addgene_12151), pTriEx-RhoA FLARE.sc Biosensor T19N (RRID:Addgene_12152), pRK5-myc-RhoA WT (RRID:Addgene_12962), pRK5-mycRhoA Q63L (RRID:Addgene_12964), pRK5-myc-RhoA T19N (RRID:Addgene_12963), EGFP-p65 (RRID:Addgene_111190), GW1-pHRed (RRID:Addgene_31473), GW1CMV-Perceval (RRID:Addgene_21737), Laconic/pcDNA3.1 (+) (RRID:Addgene_118627), Pyronic /pcDNA3.1 (+) (RRID:Addgene_51308), pcDNA3.1 FLII12Pglu-700uDelta6 (RRID:Addgene_17866), Cyto-ABKAR (RRID:Addgene_61510), pLentiEKAR2G2 (RRID:Addgene_40178), Kras-Src FRET biosensor (RRID:Addgene_78302), pFRET-HSP33 cys (RRID:Addgene_16076), pGP-CMV-GCaMP6F (RRID:Addgene_40755), mCherry-Lifeact-7 (RRID:Addgene_54491), pMitoTimer (RRID:Addgene_52659), pCLBW cox8 EGFP mCherry (RRID:Addgene_78520).

Techniques: Labeling, Cell Counting, Expressing

Fig. 2 RhoA regulates microglial metabolic reprogramming during inflammation. HMC3 microglia expressing the ATP biosensor (A), Glucose biosensor (B), Lactate biosensor (C), or Pyruvate biosensor (D) were transfected with RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 15-30 cells per group from 3 independent experiments for each biosensor). Primary cortical microglia expressing the ATP biosensor (E) or Lactate biosensor (F) were transfected with the RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 6 cells per group from 3 independent experiments for each biosensor). Panels are time-lapse ratio images coded according to the pseudocolor ramps. Graphs (means and SD) display F490/F435 (A and E), FRET/Donor (B), and Donor/FRET (C, D, and F) ratio change at 0 (CT) and 20 min. G Seahorse measurements of bioenergetic parameters in HCM3 microglia expressing RhoA Q63L or RhoA WT. The parameters were calculated based on the OCR following the sequential addition of LPS, oligomycin, FCCP, rotenone, and antimycin A. Results are from at least 3 independent experiments. Graphs show the mean with SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.

Journal: Cell death & disease

Article Title: RhoA balances microglial reactivity and survival during neuroinflammation.

doi: 10.1038/s41419-023-06217-w

Figure Lengend Snippet: Fig. 2 RhoA regulates microglial metabolic reprogramming during inflammation. HMC3 microglia expressing the ATP biosensor (A), Glucose biosensor (B), Lactate biosensor (C), or Pyruvate biosensor (D) were transfected with RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 15-30 cells per group from 3 independent experiments for each biosensor). Primary cortical microglia expressing the ATP biosensor (E) or Lactate biosensor (F) were transfected with the RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 6 cells per group from 3 independent experiments for each biosensor). Panels are time-lapse ratio images coded according to the pseudocolor ramps. Graphs (means and SD) display F490/F435 (A and E), FRET/Donor (B), and Donor/FRET (C, D, and F) ratio change at 0 (CT) and 20 min. G Seahorse measurements of bioenergetic parameters in HCM3 microglia expressing RhoA Q63L or RhoA WT. The parameters were calculated based on the OCR following the sequential addition of LPS, oligomycin, FCCP, rotenone, and antimycin A. Results are from at least 3 independent experiments. Graphs show the mean with SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.

Article Snippet: Raichu-RhoA (provided by M. Matsuda [27]), pTriEx-RhoA FLARE.sc Biosensor WT (RRID:Addgene_12150), pTriEx-RhoA FLARE.sc Biosensor Q63L (RRID:Addgene_12151), pTriEx-RhoA FLARE.sc Biosensor T19N (RRID:Addgene_12152), pRK5-myc-RhoA WT (RRID:Addgene_12962), pRK5-mycRhoA Q63L (RRID:Addgene_12964), pRK5-myc-RhoA T19N (RRID:Addgene_12963), EGFP-p65 (RRID:Addgene_111190), GW1-pHRed (RRID:Addgene_31473), GW1CMV-Perceval (RRID:Addgene_21737), Laconic/pcDNA3.1 (+) (RRID:Addgene_118627), Pyronic /pcDNA3.1 (+) (RRID:Addgene_51308), pcDNA3.1 FLII12Pglu-700uDelta6 (RRID:Addgene_17866), Cyto-ABKAR (RRID:Addgene_61510), pLentiEKAR2G2 (RRID:Addgene_40178), Kras-Src FRET biosensor (RRID:Addgene_78302), pFRET-HSP33 cys (RRID:Addgene_16076), pGP-CMV-GCaMP6F (RRID:Addgene_40755), mCherry-Lifeact-7 (RRID:Addgene_54491), pMitoTimer (RRID:Addgene_52659), pCLBW cox8 EGFP mCherry (RRID:Addgene_78520).

Techniques: Expressing, Transfection

Fig. 3 RhoA regulates microglial proinflammatory reactivity. HMC3 microglia expressing a ROS biosensor (A), AMPK biosensor (B), ERK biosensor (C), Src biosensor (D), and GFP-tagged p65 NFkB subunit (E) were transfected with the RhoA Q63L construct or with RhoA WT and then exposed to LPS (1 µg/ml for 20 min) (n = 18–100 cells per group from 3 independent experiments for each biosensor). Panels show time- lapse ratio images or mean fluorescent intensity (MFI) coded according to the pseudocolor ramps. F ELISA (TNF-α or IL-1ß) from culture supernatants of primary cortical microglia transfected with RhoA Q63L or RhoA WT and exposed to LPS (1 µg/ml) for 3 h (n = 4 independent experiments). Graphs are means with SD. *p < 0.05, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.

Journal: Cell death & disease

Article Title: RhoA balances microglial reactivity and survival during neuroinflammation.

doi: 10.1038/s41419-023-06217-w

Figure Lengend Snippet: Fig. 3 RhoA regulates microglial proinflammatory reactivity. HMC3 microglia expressing a ROS biosensor (A), AMPK biosensor (B), ERK biosensor (C), Src biosensor (D), and GFP-tagged p65 NFkB subunit (E) were transfected with the RhoA Q63L construct or with RhoA WT and then exposed to LPS (1 µg/ml for 20 min) (n = 18–100 cells per group from 3 independent experiments for each biosensor). Panels show time- lapse ratio images or mean fluorescent intensity (MFI) coded according to the pseudocolor ramps. F ELISA (TNF-α or IL-1ß) from culture supernatants of primary cortical microglia transfected with RhoA Q63L or RhoA WT and exposed to LPS (1 µg/ml) for 3 h (n = 4 independent experiments). Graphs are means with SD. *p < 0.05, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.

Article Snippet: Raichu-RhoA (provided by M. Matsuda [27]), pTriEx-RhoA FLARE.sc Biosensor WT (RRID:Addgene_12150), pTriEx-RhoA FLARE.sc Biosensor Q63L (RRID:Addgene_12151), pTriEx-RhoA FLARE.sc Biosensor T19N (RRID:Addgene_12152), pRK5-myc-RhoA WT (RRID:Addgene_12962), pRK5-mycRhoA Q63L (RRID:Addgene_12964), pRK5-myc-RhoA T19N (RRID:Addgene_12963), EGFP-p65 (RRID:Addgene_111190), GW1-pHRed (RRID:Addgene_31473), GW1CMV-Perceval (RRID:Addgene_21737), Laconic/pcDNA3.1 (+) (RRID:Addgene_118627), Pyronic /pcDNA3.1 (+) (RRID:Addgene_51308), pcDNA3.1 FLII12Pglu-700uDelta6 (RRID:Addgene_17866), Cyto-ABKAR (RRID:Addgene_61510), pLentiEKAR2G2 (RRID:Addgene_40178), Kras-Src FRET biosensor (RRID:Addgene_78302), pFRET-HSP33 cys (RRID:Addgene_16076), pGP-CMV-GCaMP6F (RRID:Addgene_40755), mCherry-Lifeact-7 (RRID:Addgene_54491), pMitoTimer (RRID:Addgene_52659), pCLBW cox8 EGFP mCherry (RRID:Addgene_78520).

Techniques: Expressing, Transfection, Construct, Enzyme-linked Immunosorbent Assay

Fig. 5 Inflammation disrupts Ca2+, pH, and mitochondrial homeostasis in RhoA-deficient microglia. A Lifeact fluorescence labeling in RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia (n = 3 independent experiments). B Cell counting in RhoA WT, RhoA Q63L, RhoA T19N, or RhoA KO HMC3 microglia (n = 8 independent experiments). Graph displays mean with SD. ****p < 0.0001 (Two-way ANOVA). RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia expressing a global Ca2+ biosensor (C), mitochondrial Ca2+ biosensor (D), endoplasmic reticulum Ca2+ biosensor (E), pH biosensor (F), MitoTimer biosensor (G), or mitophagy biosensor (H) and exposed to LPS (1 µg/ml; 20 min (C-F) or 60 min (G and H)). Graphs (mean and SD) display fluorescence changes (n = 15 cells per group from 3 independent experiments for each biosensor). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.

Journal: Cell death & disease

Article Title: RhoA balances microglial reactivity and survival during neuroinflammation.

doi: 10.1038/s41419-023-06217-w

Figure Lengend Snippet: Fig. 5 Inflammation disrupts Ca2+, pH, and mitochondrial homeostasis in RhoA-deficient microglia. A Lifeact fluorescence labeling in RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia (n = 3 independent experiments). B Cell counting in RhoA WT, RhoA Q63L, RhoA T19N, or RhoA KO HMC3 microglia (n = 8 independent experiments). Graph displays mean with SD. ****p < 0.0001 (Two-way ANOVA). RhoA WT, RhoA T19N, or RhoA KO HMC3 microglia expressing a global Ca2+ biosensor (C), mitochondrial Ca2+ biosensor (D), endoplasmic reticulum Ca2+ biosensor (E), pH biosensor (F), MitoTimer biosensor (G), or mitophagy biosensor (H) and exposed to LPS (1 µg/ml; 20 min (C-F) or 60 min (G and H)). Graphs (mean and SD) display fluorescence changes (n = 15 cells per group from 3 independent experiments for each biosensor). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.

Article Snippet: Raichu-RhoA (provided by M. Matsuda [27]), pTriEx-RhoA FLARE.sc Biosensor WT (RRID:Addgene_12150), pTriEx-RhoA FLARE.sc Biosensor Q63L (RRID:Addgene_12151), pTriEx-RhoA FLARE.sc Biosensor T19N (RRID:Addgene_12152), pRK5-myc-RhoA WT (RRID:Addgene_12962), pRK5-mycRhoA Q63L (RRID:Addgene_12964), pRK5-myc-RhoA T19N (RRID:Addgene_12963), EGFP-p65 (RRID:Addgene_111190), GW1-pHRed (RRID:Addgene_31473), GW1CMV-Perceval (RRID:Addgene_21737), Laconic/pcDNA3.1 (+) (RRID:Addgene_118627), Pyronic /pcDNA3.1 (+) (RRID:Addgene_51308), pcDNA3.1 FLII12Pglu-700uDelta6 (RRID:Addgene_17866), Cyto-ABKAR (RRID:Addgene_61510), pLentiEKAR2G2 (RRID:Addgene_40178), Kras-Src FRET biosensor (RRID:Addgene_78302), pFRET-HSP33 cys (RRID:Addgene_16076), pGP-CMV-GCaMP6F (RRID:Addgene_40755), mCherry-Lifeact-7 (RRID:Addgene_54491), pMitoTimer (RRID:Addgene_52659), pCLBW cox8 EGFP mCherry (RRID:Addgene_78520).

Techniques: Labeling, Cell Counting, Expressing